TransNGS® CUT&Tag Library Prep Kit for Illumina®

SKU: KP172-01

Product Details

The kit is designed to prepare CUT&Tag libraries for mammalian cells for the Illumina high-throughput sequencing platform. The prepared libraries can be used for Single-end sequencing and Paired-end sequencing. Cleavage Under Target & Tagmentation (CUT&Tag) is a new method for studying protein interactions with DNA. Protein A/G-Tn5 transposase fusion protein and the target protein can form an immune complex through antibody bridging. Tn5 transposase is activated in the presence of Mg²⁺, and so short sequences containing adapters can be inserted into both ends of the DNA fragment that binds to the target protein. Finally, library can be constructed by PCR amplification. The kit is suitable for DNA research on the interaction of DNA-binding proteins such as transcription factors and co-regulators. Compared with traditional ChIP-seq, it has the advantages of more accurate and stable protein-DNA interaction information in low input of cells.

Applications

10-10⁵ mammalian cells, or processed cell nucleus, and eukaryotic cells without cell wall (such as plant cell protoplasts, etc.).

Storage

Box 1 is stored at 2℃-8℃ for 1 year and Box 2 is stored at -18℃ or below for 1 year.

Shipping

Dry ice (-70℃)

Product Composition

	Component	KP172-01 (12 rxns)	KP172-02 (48 rxns)
Box1	ConA Magnetic Beads II	60 μl	240 μl
	CUT&Tag DNA Clean Beads	2 ml	4 ml × 2
Box2	ConA Beads Binding Buffer	660 μl × 2	2.7 ml × 2
	Cell Wash Buffer	10 ml × 3	40 ml × 3
	Nuclear Extraction Buffer	6 ml	6 ml × 4
	1×Pro-Wash Buffer	10 ml	40 ml
	pAG-Tn5	12 μl	48 μl
	Tagmentation Buffer	600 μl	2.4 ml
	Protease Inhibitor	420 μl	840 μl × 2
	Ab Enhancer	20 μl	80 μl
	Binding Enhancer	80 μl	320 μl
	500 mM EDTA	30 μl	120 μl
	Lysis Enhancer	20 μl	80 μl
	Proteinase K（20 mg/ml）	20 μl	80 μl
	2×CUT&Tag Library Amplification Mix	300 μl	600 μl × 2
	Nuclease-free Water	1 ml	4 ml