Trans10 Chemically Competent Cell

SKU: CD101-01

Description	Trans10 Chemically Competent Cell is specifically designed for chemical transformation of DNA. It permits a transformation efficiency of over 108 cfu/μg DNA (tested by pUC19 plasmid DNA). The competent cell is resistant to streptomycin sulfate (StrR).
Genotype	F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80 lacZΔM15Δ lacX74 recA1 araΔ139Δ(ara-leu)7697 galU galKrpsL (StrR) endA1 nupG
Features	• High transformation efficiency: >108 cfu/μg ( pUC19 DNA). • StrR. • Blue/white selection. • Toxic gene cloning and stable replication of plasmid DNA.
Storage	at -70 °C for six months
Notes	Higher efficiency transformation can be achieved by transforming cells immediately following thawing. Avoid repeated thawing. Gentle handling is required for the entire procedure.

References:

Literature	Journal	IF	Author	Date	Link
MAPK signaling pathway alters expression of midgut ALP and ABCC genes and causes resistance to Bacillus thuringiensis Cry1Ac toxin in diamondback moth	PLoS Genetics	6.6609	Zhaojiang Guo	2015 Apr	The original link
Improved flux and anti-biofouling performances of reverse osmosis membrane via surface layer-bylayer assembly	journal of membrane science	6.0349	Yao Wang,et al.	2017Oct	The original link
Producing aglycons of ginsenosides in bakers' yeast	Scientific Reports	5.5779	Zhubo Dai, et al. Tianjin Institute of Industrial	2014 Jan	The original link
Dual sgRNA-directed gene knockout using CRISPR/Cas9 technology in Caenorhabditis elegans	Scientific Reports	5.5779	Chen X, et al. School of Life Sciences, University	2014 Dec	The original link
Heterologous Expression of Helicoverpa armigera Cytochrome P450 CYP6B7 in Pichia pastoris and Interactions of CYP6B7 with Insecticides	pest management science	3.253	Chunqing Zhao,et al.	2017Mar	The original link