T7 Endonuclease I

SKU: LE101-01

Product Details

T7 Endonuclease I is a junction-resolving enzyme of 149 amino acid residue, encoded by gene 3 of bacteriophage T7, existing as a stable dimer. Not only does it selectively bind and cleave four-way DNA (Holliday) junctions with high specificity for branched structures in double-stranded DNA, such as cruciform DNA, but also it has a strong preference for cutting single-stranded DNA. It requires metal ions such as magnesium for activity. This product is purified from E. coli expressing the recombinant T7 Endonuclease I (T7EI) gene.

Definition of Activity Unit
One unit is defined as the amount of enzyme required to convert > 90% of 1 μg of supercoiled cruciform pUC(AT) to > 90% linear form in a total reaction volume of 50 μl in 1 hour at 37°C.

Applications
• Gene mutation and SNP detection for result from TALEN and CRISPR/CAS9
• Recognition and cleavage for non-perfectly matched DNA and Holliday junctions
• Randomly cleave single-stranded DNA

Storage
at -20°C for one year

Shipping
Dry ice (-70 ℃)

Product Contents

Component	LE101-01	LE101-02
T7 Endonuclease I	250 units	5×250 units
10×T7 Endonuclease I Buffer	200 μl	1 ml
T7 Endonuclease I Control Template (40 ng/μl)	20 μl	20 μl
10×DNA Loading Buffer	1 ml	1 ml