GeneBio Systems

SIRT1 ELISA kit (Human)

Name: SIRT1 ELISA kit (Human)
Brand: GeneBio Systems
SKU: SEE912Hu
Price: 996.95 EUR
Availability: InStock

SKU:SEE912Hu

€996,95 EUR

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Quantity

Size: 96Tests

# of Times Cited in literature: 23

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: SIRT1

Target Full Name: Sirtuin 1

Alternative Names: SIR2L1; Silent Mating Type Information Regulation 2 Homolog 1; NAD-Dependent Deacetylase Sirtuin-1; SIR2-like protein 1; SirtT1 75 kDa fragment

Target Species: Human

Uniprot: Q96EB6

Gene ID: 23411

Featured Series: SE kit

Featured Series Function: Detects protein (regular version)

Specificity: Reactive with Human SIRT1 / Sirtuin 1

Method: Colormetric

Detection principle: Double-antibody Sandwich

Detection range: 0.78-50ng/mL

Sensitivity: 0.29ng/mL

Assay Time: 3h

Sample Size: 100uL

Recommended/Predicted Sample Types: Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sirtuin 1 (SIRT1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sirtuin 1 (SIRT1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Sirtuin 1 (SIRT1). No significant cross-reactivity or interference between Sirtuin 1 (SIRT1) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sirtuin 1 (SIRT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sirtuin 1 (SIRT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sirtuin 1 (SIRT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sirtuin 1 (SIRT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Research Area: Endocrinology;

References Citing This Product: SIRT1 Gene Polymorphisms Affect the Protein Expression in Cardiovascular Diseases

Serum miR-206 and miR-132 as Potential Circulating Biomarkers for Mild Cognitive Impairment

Increases in peripheral SIRT1: A new biological characteristic of asthma

A Remarkable Age-Related Increase in SIRT1 Protein Expression against Oxidative Stress in Elderly:

Changes of serum Mir-217 and the correlation with the severity in type 2 diabetes patients with different stages of diabetic kidney disease.

A sirtuin 1/MMP2 prognostic index for myocardial infarction in patients with advanced coronary artery disease

Serum concentrations and gene expression of sirtuin 1 in healthy and slightly overweight subjects after caloric restriction or resveratrol supplementation: A randomized trial

Association of SIRT1 gene polymorphism and its expression for the risk of alcoholic fatty liver disease in the Han population

Gene Expression of Sirtuin-1 and Endogenous Secretory Receptor for Advanced Glycation End Products in Healthy and Slightly Overweight Subjects after …

Specific alterations in the circulating levels of the SIRT1, TLR4, and IL7 proteins in patients with dementia

The Kidney Specific Protein myo-Inositol Oxygenase, a Potential Biomarker for Diabetic Nephropathy