Simian OCLN(Occludin) ELISA Kit

SKU: ELK11642

Size:96T

Reactivity:Simian

Alternative Names:Tight Junction Protein Occludin TM4 Minus

Assay Type:Sandwich

Sensitivity:0.054 ng/mL

Standard:10 ng/mL

Detection range:0.16-10 ng/mL

Sample type:tissue homogenates, cell lysates and other biological fluids

Assay length:3.5h

Research Area:Signal transduction;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian OCLN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian OCLN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian OCLN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian OCLN in the samples is then determined by comparing the OD of the samples to the standard curve.

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