Simian ICOSLG(Inducible T-Cell Co Stimulator Ligand) ELISA Kit

SKU: ELK11486

Size:96T

Reactivity:Simian

Alternative Names:CD275; B7-H2; B7H2; B7RP-1; B7RP1; GL50; ICOS-L; ICOSL; LICOS; B7-like protein Gl50; B7-related protein 1

Assay Type:Sandwich

Sensitivity:0.067 ng/mL

Standard:10 ng/mL

Detection range:0.16-10 ng/mL

Sample type:serum, tissue homogenates, cell lysates and other biological fluids

Assay length:3.5h

Research Area:Signal transduction;Tumor immunity;Infection immunity;Immune molecule;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian ICOSLG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian ICOSLG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian ICOSLG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian ICOSLG in the samples is then determined by comparing the OD of the samples to the standard curve.

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