Simian GAD2(Glutamate Decarboxylase 2, Acid) ELISA Kit

SKU: ELK11712

Size:96T

Reactivity:Simian

Alternative Names:GAD65; Pancreatic Islets And Brain,65kDa; 65 kDa glutamic acid decarboxylase; Glutamate decarboxylase 65 kDa isoform

Assay Type:Sandwich

Sensitivity:0.28 ng/mL

Standard:50 ng/mL

Detection range:0.79-50 ng/mL

Sample type:tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length:3.5h

Research Area:Enzyme & Kinase;Endocrinology;Neuro science;Autoimmunity;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian GAD2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian GAD2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian GAD2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian GAD2 in the samples is then determined by comparing the OD of the samples to the standard curve.

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