Simian CA2(Carbonic Anhydrase II) ELISA Kit

SKU: ELK6774

Size:96T

Reactivity:Simian

Alternative Names:CA-II; CAII; Car2; CAC; Carbonate dehydratase II; Carbonic anhydrase C

Assay Type:Sandwich

Sensitivity:1.27 ng/mL

Standard:200 ng/mL

Detection range:3.13-200 ng/mL

Sample type:Serum, plasma and other biological fluids.

Assay length:3.5h

Research Area:Enzyme & Kinase;Tumor immunity;Bone metabolism;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian CA2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian CA2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian CA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian CA2 in the samples is then determined by comparing the OD of the samples to the standard curve.

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