S100A7 ELISA kit (Human)

SKU: SEC035Hu

Size: 96Tests

# of Times Cited in literature: 9

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: S100A7

Target Full Name: S100 Calcium Binding Protein A7

Alternative Names: S100-A7; PSOR1; S100A7c; Psoriasin 1

Target Species: Human

Uniprot: P31151

Gene ID: 6278

Featured Series: SE kit

Featured Series Function: Detects protein (regular version)

Specificity: Reactive with Human S100A7 / S100 Calcium Binding Protein A7

Method: Colormetric

Detection principle: Double-antibody Sandwich

Detection range: 125-8,000pg/mL

Sensitivity: 50pg/mL

Assay Time: 3h

Sample Size: 100uL

Recommended/Predicted Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level S100 Calcium Binding Protein A7 (S100A7) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level S100 Calcium Binding Protein A7 (S100A7) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of S100 Calcium Binding Protein A7 (S100A7). No significant cross-reactivity or interference between S100 Calcium Binding Protein A7 (S100A7) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to S100 Calcium Binding Protein A7 (S100A7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to S100 Calcium Binding Protein A7 (S100A7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain S100 Calcium Binding Protein A7 (S100A7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of S100 Calcium Binding Protein A7 (S100A7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Research Area: Tumor immunity;Infection immunity;

References Citing This Product: Serum levels of antimicrobial peptides and proteins do not correlate with psoriasis severity and are increased after treatment with fumaric acid esters

Proteomic Signature of Endothelial Dysfunction Identified in the Serum of Acute Ischemic Stroke Patients by the iTRAQ-Based LC–MS Approach

S100A7 (psoriasin) inhibits human epidermal differentiation by enhanced IL-6 secretion through IκB/NF-κB signaling

Plasma proteins as potential targets of abnormal Savda syndrome in asthma patients treated with unique Uighur prescription

The psoriasis-associated IL-17A induces and cooperates with IL-36 cytokines to control keratinocyte differentiation and function.

A potential contribution of psoriasin to vascular and epithelial abnormalities and inflammation in systemic sclerosis

Measurements of AMPs in stratum corneum of atopic dermatitis and healthy skin–tape stripping technique

Comprehensive proteomic profiling of patients' tears identifies potential biomarkers for the traumatic vegetative state

Salivary proteins from dysplastic leukoplakia and oral squamous cell carcinoma and their potential for early detection

Alarmins HMGB1, IL-33, S100A7, and S100A12 in Psoriasis Vulgaris