Recombinant ZIKV (strain Zika SPH2015) Envelope protein ( His Tag)

SKU: PKSV030266

Size: 100μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Viral Proteins

Abbreviation:

Target Synonym: E protein;Zika virus;strain Zika SPH2015

Research Areas:

Conjugation:

Target Species: ZIKV

Expression Host: Baculovirus-Insect Cells

Application:

Fusion tag: C-His

UNIProt ID:

Accession: ALU33341.1

Background: Envelope of Zika virus is resposible for receptor binding and membrane. Analysis of the envelope protein of Zika, from Brazilian Zika SPH215 (KU321639), indicates predicted B and T cell epitopes in peptides that are consistent to those reported for dengue, YFYF and Japanese encephalitis. The envelope Domain II B cell epitope, to which much dengue non-neutralizing cross reaction is attributed, is also conserved also in Zika virus, consistent with prior field observations of cross reactivity with dengue and YF.Domain III of the Zika envelope protein, likely the main specific neutralizing domain, is distinct from recent Brazilian dengue isolates and a recent Peruvian YF isolate (GQ379163), 76% of possible major histocompatibility complex class (MHC) I and MHC II binding peptides and potential B cell linear epitopes are unique to Zika virus.

Concentration:

Activity: Not validated for activity

Sequence: Ile291-Gly694

Purity: > 90 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 8.0, 10 % glycerol.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 45.5 kDa.

ObservedMW: