Recombinant TNF-alpha Monoclonal Antibody

SKU: AN300581P

Size::

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Shipping:Ice bag

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Recombinant Antibodies;Monoclonal Antibodies

Abbreviation:TNF

Target Synonym:MGC;Tnfsf;TNF-a;Cachectin;DIF;ICD1;ICD2;Membrane Form;MGC124630;N-terminal fragment;NTF;RATTNF;Soluble Form;TNF-alpha;Tumor Necrosis Factor;Tumor Necrosis Factor Ligand Superfamily Member 2;Tnf;Tnfa;Tnfsf2

Research Areas:

Conjugation:Unconjugated

Host:Rabbit

Species reactivity:Rat

Application:ICC/IF

Isotype:IgG

Clonality:Monoclonal

Clone NO.:11A12

UNIProt ID:P16599

Accession:

Background:TNF-α is secreted by macrophages, monocytes, neutrophils, T-cells, and NK-cells. Many transformed cell lines also secrete TNF-α. Monomeric mouse TNF-α is 156 amino acid protein (N-glycosylated) with a reported molecular weight of 17.5 kD protein. TNF-α forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.

Concentration:1 mg/mL

Immunogen:Recombinant Rat TNF-alpha Protein

Buffer:0.2 μm filtered solution in PBS

Purification method:Protein A

Dilution:ICC/IF 1:20-1:100

Calculated MW:

ObservedMW: