Recombinant TMS1/ASC Monoclonal Antibody

SKU: AN301903L

Size::50μL

Storage:Store at -20℃ Valid for 12 months. Avoid freeze / thaw cycles.

Shipping:Ice bag

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Monoclonal Antibodies;Recombinant Antibodies

Abbreviation:TMS1/ASC

Target Synonym:CARD;ASC / TMS;PYCARD;ASC;CARD5;TMS;TMS-1;TMS1;PYD and CARD domain containing;ASC / TMS1;Apoptosis associated speck like protein containing a CARD;Apoptosis-associated speck-like protein containing a CARD;CARD 5;Caspase recruitment domain containing protein 5;Caspase recruitment domain protein 5;Caspase recruitment domain-containing protein 5;hASC;MGC10332;PYD and CARD domain containing protein;PYD and CARD domain-containing protein;Target of methylation induced silencing 1;Target of methylation-induced silencing 1;TMS 1

Research Areas:Cell Biology;Cancer

Conjugation:Unconjugated

Host:Rabbit

Species reactivity:Human;

Application:WB;IHC;IF

Isotype:IgG,κ

Clonality:Monoclonal;Recombinant

Clone NO.:A619

UNIProt ID:Q9ULZ3

Accession:

Background:Target of methylation-induced silencing (TMS1)/Apoptosis-associated speck-like protein containing a CARD (ASC), also referred to as PYCARD and CARD5, is a 22-kDa pro-apoptotic protein containing an N-terminal pyrin domain (PYD) and a C-terminal caspase recruitment domain (CARD). The ASC/TMS1 gene was originally found to be aberrantly methylated and silenced in breast cancer cells, and has since been found to be silenced in a number of other cancers, including ovarian cancer, glioblastoma, melanoma, gastric cancer, lung cancer, and prostate cancer. Expression of ASC/TMS1 can be induced by pro-apoptotic/inflammatory stimuli. During apoptosis ASC/TMS1 is re-distributed from the cytosol to the mitochondria and associates with mitochondrial Bax to trigger cytochrome c release and subsequent apoptosis. ASC/TMS1 has also been found to be a critical component of inflammatory signaling where it associates with and activates caspase-1 in response to pro-inflammatory signals.

Concentration:1 mg/mL

Immunogen:Recombinant human TMS1/ASC fragment

Buffer:PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Purification method:Protein A purified

Dilution:WB 1:1000;IHC 1:100-1:500;IF 1:50

Calculated MW:22, 15 kDa

ObservedMW:22, 15 kDa