Recombinant Swine IL-6 protein(His Tag)

SKU: PKSS000005

Size: 20μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Cytokines

Abbreviation: IL-6

Target Synonym: MGI;IFNB;IL1F;POIL;IFN-β;Aldesleukin;B-Cell Differentiation Factor (BCDF);BSF2;BSF-2;catabolin;CDF;HGF;HPGF;HSF;IFNB2;IFN-β2;IL1B;IL1B1;IL1-BETA;IL1F2;IL2;Lymphokine;MGI-2;POIL2;T-cell Growth Factor (TCGF);TCGF;Interleukin-6;IL6;IL-6;Interleukin 6

Research Areas:

Conjugation:

Target Species: Porcine

Expression Host: E.coli

Application: Cell culture

Fusion tag: C-His

UNIProt ID: P26893

Accession: P26893

Background: Interleukin-6 (IL-6) is a multifunctional α-helical cytokine that regulates cell growth and differentiation of various tissues, which is known particularly for its role in the immune response and acute phase reactions. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. It exerts actions through the its heterodimeric receptor composed of IL-6R that lacks the tyrosine/kinase domain and binds IL-6 with low affinity, and ubiquitously expressed glycoprotein 130 (gp130) that binds the IL-6. IL-6R complex with high affinity and thus transduces signals. IL-6 is also involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.

Concentration:

Activity: Measure by its ability to induce proliferation in T1165.85.2.1 cells.The ED₅₀ for this effect is <1.5 ng/mL.

Sequence: Arg 31-Met 212

Purity: > 98 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method.

Calculated MW: 21.9 kDa

ObservedMW: 23 kDa