Recombinant SerpinD1/HCII Monoclonal Antibody

SKU: AN300478P

Size::

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Shipping:Ice bag

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Recombinant Antibodies;Monoclonal Antibodies

Abbreviation:SerpinD1

Target Synonym:Hcf;Heparin cofactor;Protease inhibitor leuserpin;SERPIND;Serpin D;HC-II;Heparin cofactor 2;Heparin cofactor II;Protease inhibitor leuserpin-2;Serpin D1;Hcf2;Hcii;Serpind1;HC II;HC2;HLS2;LS2;Serpin Peptidase Inhibitor Clade D Member 1;THPH10

Research Areas:

Conjugation:Unconjugated

Host:Rabbit

Species reactivity:Mouse

Application:WB;IP

Isotype:IgG

Clonality:Monoclonal

Clone NO.:5C1

UNIProt ID:P49182

Accession:

Background:SerpinD1, also known as heparin cofactor II, is a member of Serpin superfamily of the serine proteinase inhibitors. HCII is a glycoprotein in human plasma that inhibits thrombin and chymotrypsin, and the rate of inhibition of thrombin is rapidly increased by Dermatan sulfate (DS), heparin (H) and glycosaminoglycans(GAG). The stimulatory effect of glycosaminoglycans on the inhibition is mediated, in part, by the N-terminal acidic domain of HCII. Interestingly, a C-terminal His-tagged recombinant HCII exhibits enhanced activity of thrombin inhibition. It has been suggested that HCII plays an unique and important role in vascular homeostasis, and accordingly mutations in this gene or congenital HCII deficiency is potentially associated with thrombosis. HCII specifically inhibits thrombin action at the site of vascular wall injury and HCII-thrombin complexes have been detected in human plasma. HCII protects against thrombin-induced vascular remodeling in both humans and mice and HCII is a predictive biomarker and therapeutic target for atherosclerosis. SerpinD1 also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner.

Concentration:1 mg/mL

Immunogen:Recombinant Mouse SerpinD1 protein

Buffer:0.2 μm filtered solution in PBS

Purification method:Protein A

Dilution:WB 1:500-1:1000;IP 0.2-1 μL/mg of lysate

Calculated MW:57 kDa

ObservedMW:53 kDa