Recombinant SARS-CoV Nucleoprotein / NP Protein (His Tag)

SKU: PKSV030248

Size: 100μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Viral Proteins

Abbreviation:

Target Synonym: SARS;coronavirus NP Protein;coronavirus Nucleocapsid Protein;coronavirus Nucleoprotein Protein;cov np Protein;ncov NP Protein;novel coronavirus NP Protein;novel coronavirus Nucleocapsid Protein;novel coronavirus Nucleoprotein

Research Areas:

Conjugation:

Target Species: SARS

Expression Host: Baculovirus-Insect Cells

Application:

Fusion tag: C-His

UNIProt ID: Q6S8E1

Accession: NP_828858.1

Background: Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.

Concentration:

Activity: Not validated for activity

Sequence: Met1-Ala422

Purity: > 80 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 47.5 kDa

ObservedMW: 47.1 kDa