Recombinant SARS-CoV-2 Papain-like Protease Protein (His Tag)

SKU: PKSR030511

Size: 100μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Viral Proteins

Abbreviation:

Target Synonym: PL-PRO;PLpro;Papain-like Protease;Replicase polyprotein 1a;pp1a

Research Areas:

Conjugation:

Target Species: SARS-CoV-2

Expression Host: E.coli

Application: Enzyme

Fusion tag: N-His

UNIProt ID:

Accession: YP_009725299.1

Background: The coronaviral proteases, papain-like protease (PLpro) and 3C-like protease (3CLpro), are attractive antiviral drug targets because they are essential for coronaviral replication. PLpro has the additional function of stripping ubiquitin and ISG15 from host-cell proteins to aid coronaviruses in their evasion of the host innate immune responses. Targeting PLpro with antiviral drugs may have an advantage in not only inhibiting viral replication but also inhibiting the dysregulation of signaling cascades in infected cells that may lead to cell death in surrounding, uninfected cells.

Concentration:

Activity: Measured by its ability to cleave a fluorogenic peptide substrate, Arg-Leu-Arg-Gly-Gly-AMC (RLRGGAMC). The Specific Activity is > 80pmols/min/μg

Sequence: Glu1564-Val1880

Purity: > 90 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile 20 mM Tris 500mM NaCl, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 36.8 kDa

ObservedMW: