Recombinant Rat CNTF protein

SKU: CD00133

Size:5 ug

Background:Ciliary neurotrophic factor (CNTF) is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. CNTF was initially identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture. Furthermore, the protein is also a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. In addition, CNTF is useful for treatment of motor neuron disease and it could reduce food intake without causing hunger or stress. Recombinant rat CNTF containing 199 amino acids and it shares 83 % and 95 % a.a. sequence identity with human and Mouse CNTF.

Research_area:Immunology

Purity:> 95 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant mouse Ciliary Neurotrophic Factor

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by a cell proliferation assay using human TF-1 cells is less than 3 ng/ml, corresponding to a specific activity of > 3.3 × 14 IU/mg.

Synonyms:CNTF, Rat

Endotoxin:Less than 1 EU/μg of rRtCNTF as determined by LAL method.

Storage:-20ºC

Sequence:AFAEQTPLTL HRRDLCSRSI WLARKIRSDL TALMESYVKH QGLNKNINLD SVDGVPVAST DRWSEMTEAE RLQENLQAYR TFQGMLTKLL EDQRVHFTPT EGDFHQAIHT LMLQVSAFAY QLEELMVLLE QKIPENEADG MPATVGDGGL FEKKLWGLKV LQELSQWTVR SIHDLRVISS HQMGISALES HYGAKDKQM

uniprot accession:P2294;G3V7M6

Molecular weight:Approximately 22.7 kDa, a single non-glycosylated polypeptide chain containing 199 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in 2 × PBS, pH 7.4, 2 % trehalose.