Recombinant Rat CDNF protein

SKU: CD00137

Size:5 ug

Background:Cerebral dopamine neurotrophic factor (CDNF), also known as ARMET-like protein 1, is encoded by the CDNF gene and widely expressed in neuronal and non-neuronal tissues. The CDNF is a novel neurotrophic factor with strong trophic activity on dopaminergic neurons comparable to that of glial cell line-derived neurotrophic factor (GDNF). Furthermore, it also prevents the 6-hydroxydopamine (6-OHDA)-induced degeneration of dopaminergic neurons. By research, CDNF might be beneficial for the treatment of Parkinsons disease. Recombinant rat CDNF contains 163 amino acid residues and it shares 84 % and 87 % a.a. sequence identity with human and Mouse CDNF.

Research_area:Immunology

Purity:> 97 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Rat Cerebral Dopamine Neurotrophic Factor

Biological activity:Fully biologically active when compared to standard. It is able to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons when immobilized at 5 - 25 μg/mL on a nitrocellulose-coated microplate.

Synonyms:CDNF, Armetl1,Rat

Endotoxin:Less than 0.1 EU/μg of rRtCDNF as determined by LAL method.

Storage:-20ºC

Sequence:QGLEAGVRSR ADCEVCKEFL NRFYNSLLTR GIDFSVDTIE EELISFCADT KGKENRLCYY LGATKDSATK ILGEVTRPMS VHMPTVKICE KLKKMDSQIC ELKYEKKLDL ESVDLWKMRV AELKQILHSW GEECRACAEK HDYVNLIKEL APKYVETRPQ TEL

uniprot accession:PC5I

Molecular weight:Approximately 18.8 kDa, a single non-glycosylated polypeptide chain containing 163 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in PBS, pH 7.4.