Recombinant Rat CCl2 protein (His tag)

SKU: PDMR100017

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Others

Abbreviation:CCl2

Target Synonym:C-C motif chemokine 2;Ccl2;Immediate-early serum-responsive protein JE;MCP-1;Mcp1;Monocyte chemoattractant protein 1;Monocyte chemotactic protein 1;Scya2;Small-inducible cytokine A2

Research Areas:Cardiovascular;Cancer;immunology;metabolism

Conjugation:

Target Species:Rat

Expression Host:HEK293 Cells

Application:

Fusion tag:C-His

UNIProt ID:P14844

Accession:P14844

Background:The chemokine (C-C motif) ligand 2 (CCL2), also known as monocyte chemoattractant protein (MCP)-1 and small inducible cytokine A2 (SCYA2)), is a small cytokine that belongs to the CC chemokine family responsible for monocyte attraction. Its cognate receptor, CCR2, play a critical role in regulating nociceptive processes during neuropathic pain. Both CCL2 and CCR2 are implicated in induction of autoimmunity. CCL2 recruits monocytes, memory T cells, and dendritic cells to the sites of inflammation produced by either tissue injury or infection. Recently research also showed that CCL2 might be useful as a biomarker of fibrosis as well as a target for therapeutic intervention.

Concentration:

Activity:Not validated for activity

Sequence:Met1-Asn148

Purity: > 95% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from sterile PBS, pH 7.4.
Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin:< 1.0 EU/mg of the protein as determined by the LAL method.

Calculated MW:13.6 kDa

ObservedMW:40 kDa