Recombinant Porcine reproductive and respiratory syndrome virus Glycoprotein 5 (GP5), partial

SKU: A0MD34

Size: 100ug. Other sizes are also available.

Activity: Not tested

Research Areas: Others

Uniprot ID: A0MD34

Gene Names: GP5

Alternative Name(s): Protein GP5;GL

Abbreviation: Recombinant Porcine reproductive and respiratory syndrome virus GP5 protein, partial

Organism: Porcine reproductive and respiratory syndrome virus (isolate Pig/United States/SD 01-08/2001) (PRRSV)

Source: E.coli

Expression Region: 33-63aa

Protein Length: Partial

Tag Info: N-terminal GST-tagged

Target Protein Sequence: DGNGNNSTYQYIYNLTICELNGTNWLSGHFE

MW: 30.2 kDa

Purity: Greater than 95% as determined by SDS-PAGE.

Endotoxin: Not test

Biological_Activity:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Relevance: Major envelope protein present in abundant amounts in the virion envelope. Mediates virion sialic acid-dependent attachment the sialoadhesin receptor SIGLEC1. This attachment induces virion internalization into alveolar macrophages predominantly through clathrin-dependent endocytosis.

Reference:

Function: