Recombinant Porcine IL-2 protein

SKU: CD00153

Size:2 ug

Background:IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. It is expressed by CD4+ and CD8+ T cells, γδ T cells, B cells, dendritic cells, and eosinophils. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. The receptor for IL-2 consists of three subunits (55 kDa IL2Rα, 75 kDa IL2Rβ, 64 kDa common gamma chain γc/IL2Rγ) that are present on the cell surface in varying preformed complexes. Recombinant porcine IL-2 is a 15.3 kDa protein containing 134 amino acid residues and it shares about 72 % amino acid sequence identity with mouse, human and rat IL-2. It also shares 60 % and 67 % acid sequence identity with rhesus macaque and equus caballus IL-2, respectively.

Research_area:Immunology

Purity:> 96 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Porcine Interleukin-2

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by a cell proliferation assay using Mouse CTLL-2 cells is less than .5 ng/ml, corresponding to a specific activity of > 2. × 16 IU/mg.

Synonyms:IL-2,IL2, POIL2, TCGF, Porcine

Endotoxin:Less than 1 EU/ug of rPoIL-2 as determined by LAL method.

Storage:-20ºC

Sequence:APTSSSTKNT KKQLEPLLLD LQLLLKEVKN YENADLSRML TFKFYMPKQA TELKHLQCLV EELKALEGVL NLGQSKNSDS ANIKESMNNI NVTVLELKGS ETSFKCEYDD ETVTAVEFLN KWITFCQSIY STLT

uniprot accession:P26891;A8D5G2;Q5W7K8;G9JKK6

Molecular weight:Approximately 15.2 kDa, a single non-glycosylated polypeptide chain containing 134 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in 1 × PBS, pH 7.4.