Recombinant Plasmodium falciparum Apical membrane antigen 1 (AMA-1), partial

SKU: P22621

Size: 100ug. Other sizes are also available.

Activity: Not tested

Research Areas: Others

Uniprot ID: P22621

Gene Names: AMA-1

Alternative Name(s): Merozoite surface antigen

Abbreviation: Recombinant Plasmodium falciparum AMA-1 protein, partial

Organism: Plasmodium falciparum (isolate FC27 / Papua New Guinea)

Source: E.coli

Expression Region: 42-73aa&227-271aa&346-407aa&452-488aa&525-546aa

Protein Length: partial

Tag Info: N-terminal 6xHis-tagged

Target Protein Sequence: NEHREHPKEYQYPLHQEHTYQQEDSGEDENTLDNDKNSNYKYPAVYDDKDKKCHILYIAAQENNGPRYCNKDESKRNASDQPKQYEQHLTDYEKIKEGFKNKNASMIKSAFLPTGAFKADRYKSHGKGYNWGNYNTETQKEIERESKRIKLNDNDDEGNKKIIAPRIFISDDKDSLKEEYKDEYADIPEHKPTYDKMK

MW: 27.5 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Endotoxin: Not test

Biological_Activity:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Relevance: Involved in parasite invasion of erythrocytes.

Reference: "Integral membrane protein located in the apical complex of Plasmodium falciparum." Peterson M.G., Marshall V.M., Smythe J.A., Crewther P.E., Lew A., Silva A., Anders R.F., Kemp D.J. Mol. Cell. Biol. 9: 3151-3154(1989)

Function: Involved in parasite invasion of erythrocytes.