Recombinant Pig Transferrin receptor protein 1 (TFRC), partial

SKU: Q8HZV3

Size: 100ug. Other sizes are also available.

Activity: Not tested

Research Areas: cancer

Uniprot ID: Q8HZV3

Gene Names: TFRC

Alternative Name(s): (TR)(TfR)(TfR1)(Trfr)(CD antigen CD71)

Abbreviation: Recombinant Pig TFRC protein, partial

Organism: Sus scrofa (Pig)

Source: E.coli

Expression Region: 575-768aa

Protein Length: Partial

Tag Info: N-terminal 6xHis-tagged

Target Protein Sequence: GTTMDTYDVLSKRVPQLNRMARAAAEVAGHLVIKLTIDFELNLNYEMYNDKILSFVREMNQFRVDIREMGLSLQWLYSARGDFFRATSRLTSDYRNVETRDKFVMREINDRIMKVEYHFLSPYVSPRESPFRHIFWGSGSHTLSALVEHLKLRQKNSSAFNQTLLKNQLALATWTIQGAANALSGDIWDIDNEF

MW: 25.3 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Endotoxin: Not test

Biological_Activity:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Relevance: Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. Positively regulates T and B cell proliferation through iron uptake. Acts as a lipid sensor that regulates mitochondrial fusion by regulating activation of the JNK pathway. When dietary levels of stearate (C18: 0) are low, promotes activation of the JNK pathway, resulting in HUWE1-mediated ubiquitination and subsequent degradation of the mitofusin MFN2 and inhibition of mitochondrial fusion. When dietary levels of stearate (C18: 0) are high, TFRC stearoylation inhibits activation of the JNK pathway and thus degradation of the mitofusin MFN2.

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Function: