Recombinant Phospho-MSK1 (Ser360) Monoclonal Antibody

SKU: AN301016L

Size::50μL

Storage:Store at -20℃ Valid for 12 months. Avoid freeze / thaw cycles.

Shipping:Ice bag

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Acetyl/Phospho/Methyl Antibodies;Recombinant Antibodies;Monoclonal Antibodies

Abbreviation:MSK1

Target Synonym:MSK;MSPK;RPS6KA;RPS6KA5;MSK1;MSPK1;RLPK;90 kDa ribosomal protein S6 kinase 5;EC 2.7.11.1;KS6A5;MGC1911;Mitogen and stress activated protein kinase 1;Nuclear Mitogen And Stress Activated Protein Kinase 1;Nuclear mitogen- and stress-activated protein kinase 1;Ribosomal protein S6 kinase 90kD polypeptide 5;Ribosomal protein S6 kinase 90kDa;Ribosomal protein S6 kinase 90kDa polypeptide 5;Ribosomal Protein S6 Kinase Alpha 5;Ribosomal protein S6 kinase alpha-5;RSK Like Protein Kinase;RSKL;RSK-like protein kinase;S6K alpha 5;S6K-alpha-5

Research Areas:Signal Transduction;Epigenetics and Nuclear Signaling;Cell Biology;Metabolism

Conjugation:Unconjugated

Host:Rabbit

Species reactivity:Human;Mouse;Rat

Application:WB

Isotype:IgG,κ

Clonality:Monoclonal;Recombinant

Clone NO.:2B12

UNIProt ID:O75582

Accession:

Background:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Appears to be activated by multiple phosphorylations on threonine and serine residues. ERK1/2 and MAPK14/p38-alpha may play a role in this process.,Serine/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1). Essential role in the control of RELA transcriptional activity in response to TNF. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF), which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14).,miscellaneous:Enzyme activity requires the presence of both kinase domains.,PTM:Ser-376 and Thr-581 phosphorylation is required for kinase activity. Ser-376 and Ser-212 are autophosphorylated by the C-terminal kinase domain, and their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 2 protein kinase domains.,subcellular location:Predominantly nuclear. Partially cytoplasmic.,subunit:Forms a complex with either ERK1 or ERK2 in quiescent cells which transiently dissociates following mitogenic stimulation. Also associates with MAPK14/p38-alpha. Activated RPS6KA5 associates with and phosphorylates the NF-kappa-B p65 subunit RELA.,tissue specificity:Widely expressed with high levels in heart, brain and placenta. Less abundant in lung, kidney and liver.

Concentration:0.2 mg/mL

Immunogen:A synthetic peptide corresponding to residues around (Ser360) of Human Phospho-MSK1

Buffer:PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Purification method:Protein A

Dilution:WB 1:1000-1:5000;

Calculated MW:90 kDa

ObservedMW:90 kDa