Recombinant Mouse VEGF165 protein

SKU: CD00160

Size:2 ug

Background:Vascular Endothelial Growth Factor is a sub-family of growth factors produced by cells, which stimulates vasculogenesis and angiogenesis. VEGFs normal function is to create new blood vessels during embryonic development, new blood vessels after injury, muscle following exercise, and new vessels (collateral circulation) to bypass blocked vessels. Mouse and rat express alternately spliced isoforms of 120, 165, and 188 amino acids (a.a.) in length. Recombinant mouse VEGF165 contains 165 amino acids residues and it is a disulfide-linked homodimer. In addition, it shares 97 % a.a. sequence identity with corresponding regions of rat, 89 % with human and porcine, 88 % with bovine, and 90 % with feline, equine and canine VEGF, respectively.

Research_area:Immunology

Purity:> 95 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Mouse Vascular Endothelial Growth Factor165

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by a cell proliferation assay using human umbilical vein endothelial cells(HUVEC) is less than 5 ng/ml, corresponding to a specific activity of > 2. × 15 IU/mg.

Synonyms:VEGF165, Mouse

Endotoxin:Less than 1 EU/μg of rMuVEGF165 as determined by LAL method.

Storage:-20ºC

Sequence:MAPTTEGEQK SHEVIKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNITMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR

uniprot accession:Q731

Molecular weight:Approximately 38.8 kDa, a disulfide-linked homodimeric protein consisting of two 165 amino acid polypeptide chains.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered solution in PBS, pH 7.4.