Recombinant mouse TGF-beta 2/TGFB2 protein (His tag)

SKU: PDEM100291

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Others

Abbreviation:TGF-beta 2;TGFB2

Target Synonym:BSC-1 cell growth inhibitor;Cetermin;G-TSF;Glioblastoma-derived T-cell suppressor factor;MGC116892;Polyergin;TGF-beta-2;TGF-beta2;TGFB2;transforming growth factor beta-2

Research Areas:Signal Transduction;Cell biology;Cancer;metabolism;Stem cells

Conjugation:

Target Species:mouse

Expression Host:E.coli

Application:

Fusion tag:N-His

UNIProt ID:P27090

Accession:P27090

Background:Transforming growth factor beta 2 (TGF-β2) is a member of TGF-beta superfamily that shares a characteristic cysteine knot structure. Mice with TGF-β2 gene deletion show defects in development of cardiac, lung, craniofacial, limb, spinal column, eye, inner ear and urogenital systems. All TGF-β isoforms signal via the same heteromeric receptor complex, consisting of a ligand binding TGF-β receptor type II (TβR-II), and a TGF-β receptor type I (TβR-I). Signal transduction from the receptor to the nucleus is mediated via SMADs. TGF-β expression is found in cartilage, bone, teeth, muscle, heart, blood vessels, haematopoitic cells, lung, kidney, gut, liver, eye, ear, skin, and the nervous system.

Concentration:

Activity:Not validated for activity

Sequence:Leu 21-Arg 302

Purity: > 95% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from sterile PBS, pH 7.4.
Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin:< 10 EU/mg of the protein as determined by the LAL method.

Calculated MW:30.9 kDa

ObservedMW:38 kDa