Recombinant Mouse PSMA / FOLH1 protein (His tag)

SKU: PDEM100301

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Others

Abbreviation:PSMA;FOLH1

Target Synonym:Folate hydrolase 1;Folh1;Folylpoly-gamma-glutamate carboxypeptidase (FGCP);Glutamate carboxypeptidase 2;Glutamate carboxypeptidase II (GCPII);Membrane glutamate carboxypeptidase (mGCP);N-acetylated-alpha-linked acidic dipeptidase I (NAALADase I)

Research Areas:Cancer;Metabolism;Signal Transduction

Conjugation:

Target Species:Mouse

Expression Host:E.coli

Application:

Fusion tag:N-His

UNIProt ID:O35409

Accession:O35409

Background:Glutamate carboxypeptidase 2, also known as Glutamate carboxypeptidase II, Membrane glutamate carboxypeptidase, Prostate-specific membrane antigen, GCPII, PSMA, FOLH1, and NAALAD1, is a single-pass type I&nbsp,I membrane protein which belongs to the&nbsp,peptidase M28 family and M28B subfamily. FOLH1 is highly expressed in prostate epithelium. It is detected in urinary bladder, kidney, testis, ovary, fallopian tube, breast, adrenal gland, liver, esophagus, stomach, small intestine, colon, brain (at protein level), and the capillary endothelium of a variety of tumors. FOLH1 has both folate hydrolase and N-acetylated alpha linked acidic dipeptidase (NAALADase) activity. It has a preference for tri-alpha-glutamate peptides. Genetic variation in FOLH1 may be associated with low folate levels and consequent hyperhomocysteinemia. This condition can result in increased risk of cardiovascular disease, neural tube defects, and cognitive deficits. FOLH1 also shows a promising role in directed imaging and therapy of recurrent or metastatic disease.

Concentration:

Activity:Not validated for activity

Sequence:Lys 45-Phe 335

Purity: > 95% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from sterile PBS, pH 7.4.
Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin:< 10 EU/mg of the protein as determined by the LAL method.

Calculated MW:31.9 kDa

ObservedMW:38 kDa