Recombinant Mouse Interleukin-2/IL-2 Protein

SKU: PKSM041320

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Cytokines

Abbreviation: Interleukin-2;IL-2

Target Synonym: POIL;OTTHUMP;Aldesleukin;IL 2;Interleukin 2;Involved in regulation of T cell clonal expansion;Lymphokine;OTTHUMP00000164090;POIL2;T Cell Growth Factor;T-cell growth facter;Interleukin-2;IL2;TCGF;T-cell growth factor;Il-2

Research Areas:

Conjugation:

Target Species: Mouse

Expression Host: E.coli

Application: Cell culture

Fusion tag: None

UNIProt ID: P04351

Accession: P04351

Background: Interleukin 2 (IL 2), also termed T-cell growth factor, is a member of the cytokine family which includes IL-4, IL-7, IL-9, IL-15 and IL-21. Each member of this family has a four alpha helix bundle. IL-2 signals through the IL-2 receptor, a complex consisting of tree subunits, termed alpha, beta and gamma. The IL-2 R gamma is shared by cytokine receptors of all members of cytokine family. Mature mouse IL-2 shares 56% and 73% aa sequence identity with human and rat IL-2, respectively. IL-2 is produced by CD4+ T cell, CD8+ T cells, gamma δ T cells, B cells, dendritic cells and eosinophils, and plays a vital role in key function of the immune system, tolerance and immunity, primarily via its potent stimulatory activity for T cells.Thus, IL-2 may be a key cytokine in the natural suppression of autoimmunity.

Concentration:

Activity: Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells.The specific activity of Recombinant Mouse IL-2 is ≥1×10⁷ IU/mg.

Sequence: Ala21-Gln169

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM Sodium Citrate, 0.2% Tween 80, pH 3.0.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 0.01 EU per μg of the protein as determined by the LAL method.

Calculated MW: 17.4 kDa

ObservedMW: 17 kDa