Recombinant Mouse IL-3 protein

SKU: CD00363

Size:2 ug

Background:Interleukin-3 (IL-3) is a type of biological signal (cytokine) which is encoded by the IL-3 gene located on chromosome 5 and produced primarily by activated T cells beside human thymic epithelial cells, activated Mouse mast cells, Mouse keratinocytes and neurons/astrocytes. The protein acts in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. In addition, it exerts its biological activities through binding to interleukin-3 receptors included α and β subunits. The Mouse IL-3 is different from human IL-3 and contains 140 amino acids residues. Specifically, mouse and human IL-3 share low homology and have not cross species activity.

Research_area:Immunology

Purity:> 98 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Mouse Interleukin-3

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by the dose-dependent stimulation of the proliferation of Mouse M-NFS-6 cells is less than .5 ng/ml, corresponding to a specific activity of > 2 × 17 IU/mg.

Synonyms:IL-3, BPA, Csfm, Csfmu, HCGF, Il, Il3, MCGF, PSF,Mouse

Endotoxin:Less than 1 EU/μg of rMuIL-3 as determined by LAL method.

Storage:-20ºC

Sequence:DTHRLTRTLN CSSIVKEIIG KLPEPELKTD DEGPSLRNKS FRRVNLSKFV ESQGEVDPED RYVIKSNLQK LNCCLPTSAN DSALPGVFIR DLDDFRKKLR FYMVHLNDLE TVLTSRPPQP ASGSVSPNRG TVEC

uniprot accession:P1586

Molecular weight:Approximately 14.8 kDa globular protein containing 134 amino acid residues.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered solution in PBS, pH 7.4.