Recombinant Mouse FGF-9 protein

SKU: CD00452

Size:2 ug

Background:Fibroblast growth factor 9 (FGF-9) encoded by the FGF-9 gene is a member of the fibroblast growth factor (FGF) family. It plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. In addition, this protein was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells and it is a heparin-binding protein. Furthermore, FGF-9 is a monomer and interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Recombinant mouse FGF-9 is synthesized as a 208 a.a. precursor that contains a 3 a.a. signal sequence. Specifically, The mouse FGF-9 shares 99 % a. a. sequence identity with rat FGF-9.

Research_area:Immunology

Purity:> 95 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Mouse Fibroblast Growth Factor-9

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than .5 ng/ml, corresponding to a specific activity of > 2. × 16 IU/mg.

Synonyms:FGF-9, Ek, Eks, Fgf4b,FGF9,Mouse

Endotoxin:Less than 1 EU/μg of rMuFGF-9 as determined by LAL method.

Storage:-20ºC

Sequence:MPLGEVGSYF GVQDAVPFGN VPVLPVDSPV LLNDHLGQSE AGGLPRGPAV TDLDHLKGIL RRRQLYCRTG FHLEIFPNGT IQGTRKDHSR FGILEFISIA VGLVSIRGVD SGLYLGMNEK GELYGSEKLT QECVFREQFE ENWYNTYSSN LYKHVDTGRR YYVALNKDGT PREGTRTKRH QKFTHFLPRP VDPDKVPELY KDILSQS

uniprot accession:P5413;Q3TPG

Molecular weight:Approximately 23.4 kDa, a single non-glycosylated polypeptide chain containing 27 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in 2 mM Tris, 5 mM NaCl, pH 8.5.