Recombinant Mouse E-Cadherin Protein(His Tag)

SKU: PDMM100161

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Others

Abbreviation:E-Cadherin

Target Synonym:CDH;CAM 120/;AA960649;ARC-1;CAM 120/80;CD324;CDHE;Ecad;E-cad;Epithelial Cadherin;LCAM;L-CAM;Um;UVO;Uvomorulin;Cdh1;E-Cadherin; CDH1

Research Areas:

Conjugation:

Target Species:Mouse

Expression Host:Mammalian

Application:

Fusion tag:C-His

UNIProt ID:P09803

Accession:P09803

Background:Cadherins are calcium-dependent cell adhesion proteins which preferentially interact with themselves in a homophilic manner in connecting cells , and thus may contribute to the sorting of heterogeneous cell type. E-cadherin (E-Cad) , also known as CDH1 and CD324 , is a calcium-dependent cell adhesion molecule the intact function of which is crucial for the establishment and maintenance of epithelial tissue polarity and structural integrity. Mutations in CDH1 occur in diffuse type gastric cancer , lobular breast cancer , and endometrial cancer. In Human cancers , partial or complete loss of E-cadherin expression correlates with malignancy. During apoptosis or with calcium influx , E-Cad is cleaved by the metalloproteinase to produce fragments of about 38 kDa (E-CAD/CTF1) , 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3) , respectively. E-Cad has been identified as a potent invasive suppressor , as downregulation of E-cadherin expression is involved in dysfunction of the cell-cell adhesion system , and often correlates with strong invasive potential and poor prognosis of Human carcinomas.

Concentration:

Activity:Not validated for activity

Sequence:Asp157-Val709

Purity: > 90% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin:< 1.0 EU/mg of the protein as determined by the LAL method.

Calculated MW:60.7 kDa

ObservedMW:80 kDa