Recombinant Mouse CXCL2/MIP-2 Protein

SKU: PKSM040996

Size: 100μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: CXCL2;MIP-2

Target Synonym: C-X-C motif chemokine 2;GRO beta;GRO2;GROB;Gro-beta;Growth-regulated protein beta;MGSA-b;MGSA-beta;MIP-2;MIP2-alpha;MIP2A;Macrophage Inflammatory Protein-2-alpha;SCYB2.;chemokine ligand 2;cxcl2;melanoma growth stimulatory activity beta

Research Areas:

Conjugation:

Target Species: Mouse

Expression Host: E.coli

Application: Cell culture

Fusion tag: N-His

UNIProt ID: P10889

Accession: P10889

Background: C-X-C motif chemokine 2 (CXCL2,MIP-2) belongs to the intercrine alpha (chemokine CxC) family. It was originally identified as a heparin-binding protein secreted from a murine macrophage cell line in response to endotoxin stimulation. The expression of mouse MIP-2 is stimulated by endotoxin. The mouse MIP-2 shares approximately 63% aa sequence identity with murine KC, another mouse alpha chemokine, which is induced by PDGF. It has been suggested that mouse KC and MIP-2 are the homologs of the human GROs and rat CINCs. Chemotactic for human polymorphonuclear leukocytes but does not induce chemokinesis or an oxidative burst. The expression of MIP-2 was found to be associated with neutrophil influx in pulmonary inflammation and glomerulonephritis, suggesting that MIP-2 may contribute to the pathogenesis of inflammatory diseases.

Concentration:

Activity: Measure by its ability to chemoattract BaF3 cells transfected with human CXCR2.The ED₅₀ for this effect is <0.5 ng/mL.

Sequence: Ala28-Asn100

Purity: > 98 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method.

Calculated MW: 8.7 kDa

ObservedMW: 11 kDa