Recombinant Mouse CT-1 protein

SKU: CD00505

Size:2 ug

Background:Cardiotrophin-1 (CT-1) is a member of the cytokine family which also includes IL-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), and ciliary neurotrophic factor (CNTF). CT-1 is a pleiotropic cytokine which is expressed in various tissues including the adult heart, skeletal muscle, ovary, colon, prostate and fetal lung. In addition, CT-1 which induces cardiac myocyte hypertrophy in vitro can bind to and activate the ILST/gp130 receptor. Mouse CT-1 encodes a 203 amino acid (a.a.) residue protein that lacks a hydrophobic signal peptide. Human and Mouse CT-1 share 80 % a.a. sequence identity and exhibit cross-species activity.

Research_area:Immunology

Purity:> 95 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Mouse Cardiotrophin(TM)1

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by a cell proliferation assay using human TF-1 cells is less than .5 ng/ml, corresponding to a specific activity of > 2. × 16 IU/mg.

Synonyms:CT-1,CTF1, Mouse

Endotoxin:Less than 1 EU/μg of rMuCT-1 as determined by LAL method.

Storage:-20ºC

Sequence:MSQREGSLED HQTDSSISFL PHLEAKIRQT HNLARLLTKY AEQLLEEYVQ QQGEPFGLPG FSPPRLPLAG LSGPAPSHAG LPVSERLRQD AAALSVLPAL LDAVRRRQAE LNPRAPRLLR SLEDAARQVR ALGAAVETVL AALGAAARGP GPEPVTVATL FTANSTAGIF SAKVLGFHVC GLYGEWVSRT EGDLGQLVPG GVA

uniprot accession:Q8VBW1

Molecular weight:Approximately 21.5 kDa, a single non-glycosylated polypeptide chain containing 23 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in 2 mM Tris, 3 mM NaCl, pH 8.5.