Recombinant Mouse BRAK/CXCL14 protein

SKU: CD00608

Size:5 ug

Background:Chemokine (C-X-C motif) ligand 14 (CXCL14), also named BRAK, is a small cytokine belonging to the CXC chemokine family. It is constitutively expressed at the mRNA level in certain normal tissues and it possesses chemoattractive activity for activated macrophages, immature dendritic cells and natural killer cells. According to the relevant literature, CXCL14 is associated with tumor development. Moreover, recent evidence revealed that CXCL14 participates in glucose metabolism, feeding behaviour-associated neuronal circuits, and anti-microbial defense. Although CXCL14 receptors have not yet been identified, the intracellular activity of CXCL14 in breast cancer cells suggests that the CXCL14 receptor(s) and signal transduction pathway(s) may be different from those of conventional CXC-type chemokines. Recombinant Mouse CXCL14 contains 77 amino acid residues and it shares 97 % and 99 % a.a. sequence identity with human and rat CXCL14.

Research_area:Immunology

Purity:> 95 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Mouse Breast and Kindey-expressed Chemokine/CXCL14

Biological activity:Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 1.-1 ng/ml.

Synonyms:BRAK/CXCL14, 11131L23Rik, 126I23Rik, AI414372, BMAC, BR, BRAK, KS1, Kec, MIP-, MIP-2g, MIP2gamma, NJAC, Scyb1, Scyb14, bolekine,Mouse

Endotoxin:Less than 1 EU/μg of rMuBRAK/CXCL14 as determined by LAL method.

Storage:-20ºC

Sequence:SKCKCSRKGP KIRYSDVKKL EMKPKYPHCE EKMVIVTTKS MSRYRGQEHC LHPKLQSTKR FIKWYNAWNE KRRVYEE

uniprot accession:Q9WUQ5;Q6AXC2

Molecular weight:Approximately 9.4 kDa, a single non-glycosylated polypeptide chain containing 77amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in 2 mM PB, 4 mM NaCl, pH 7.4, 5 % trehalose.