Recombinant Mouse ATP-dependent RNA helicase DHX33 (Dhx33)

SKU: Q80VY9

Size: 100ug. Other sizes are also available.

Activity: Not tested

Research Areas: Cell Biology

Uniprot ID: Q80VY9

Gene Names: Dhx33

Alternative Name(s): DEAH box protein 33

Abbreviation: Recombinant Mouse Dhx33 protein

Organism: Mus musculus (Mouse)

Source: Baculovirus

Expression Region: 1-698aa

Protein Length: Full Length

Tag Info: C-terminal 6xHis-tagged

Target Protein Sequence: MPEEASLPPAKRFRPGSCPPGRRVVMLLTAGGGGGAGGGRRQTPPLAQPSASPYREALELQRRSLPIFRARGQLLAQLRNLDNAVLIGETGSGKTTQIPQYLYEGGISRQGIIAVTQPRRVAAISLATRVSDEKRTELGKLVGYTVRFEDVTSEDTRIKFLTDGMLLREAISDSLLRKYSCVILDEAHERTIHTDVLFGVVKTAQKRRKELGKLPLKVIVMSATMDVDLFSQYFNRAPVLYLEGRQHPIQIFYTKQPQQDYLHAALVSVFQIHQEAPASQDILVFLTGQEEIEAMSKTCRDIARHLPDGCPSMLVLPLYASLPYSQQLRVFQGAPKGYRKVIISTNIAETSITITGIKYVVDTGMVKAKKYNPDSGLEVLAVQRVSKTQAWQRTGRAGREDSGICYRLYTEDEFEKFEKMTVPEIQRCNLASVILQLLAMKVPNVLTFDFMSKPSPDHIEAAIAQLDLLGALEHKDDQLTLTPIGRKMAAFPLEPRFAKTILLSSKFHCTEEILTIVSLLSVDSVLYNPPARRDEVQSVRKKFISSEGDHITLLNIYRTFKNIGGNKDWCKENFVNSKNMLLVAEVRAQLREICLKMSMPIMSSRGDMESVRRCMAHSLFMNTAELQTDGTYATTDTHQPVAIHPSSVLFHCKPACVVYTSLLYTNKCYMRDLCVVDAEWLYEAAPDYFRRKLRTARN

MW: 81.2 kDa

Purity: Greater than 85% as determined by SDS-PAGE.

Endotoxin: Not test

Biological_Activity:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Relevance: Implicated in nucleolar organization, ribosome biogenesis, protein synthesis and cytoplasmic dsRNA sensing. Stimulates RNA polymerase I transcription of the 47S precursor rRNA. Associates with ribosomal DNA (rDNA) loci where it is involved in POLR1A recruitment. In the cytoplasm, promotes elongation-competent 80S ribosome assembly at the late stage of mRNA translation initiation. Senses cytosolic dsRNA mediating NLRP3 inflammasome formation in macrophages and type I interferon production in myeloid dendritic cells. Required for NLRP3 activation induced by viral dsRNA and bacterial RNA. In dendritic cells, required for induction of type I interferon production induced by cytoplasmic dsRNA via the activation of MAPK and NF-kappa-B signaling pathways.

Reference:

Function: