Recombinant Human UCHL1 protein (GST tag)

SKU: PDEH100784

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Others

Abbreviation:UCHL1

Target Synonym:HEL-117;NDGOA;Neuron Cytoplasmic Protein 9.5;PARK5;PGP 9.5;PGP9.5;UCH-L1;UCHL1;Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1;Ubiquitin Thioesterase L1;Uch-L1

Research Areas:Tags & Cell Markers;Cell biology;Neuroscience

Conjugation:

Target Species:Human

Expression Host:E.coli

Application:

Fusion tag:N-GST

UNIProt ID:P09936

Accession:P09936

Background:Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1 (UCHL1) belongs to the Peptidase C12 family. UCHL1 is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. UCHL1 is a component of the ubiquitin system, which has a fundamental role in regulating various biological activities. UCHL1 is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. UCHL1 also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer of UCHL1 may have ATP-independent ubiquitin ligase activity.

Concentration:

Activity:Not validated for activity

Sequence:Met1-Cys220

Purity: > 95% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from sterile PBS, pH 7.4.
Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin:< 10 EU/mg of the protein as determined by the LAL method.

Calculated MW:49.1 kDa

ObservedMW:30 kDa