Recombinant Human TNF- Alpha protein (N-6His)

SKU: CD00812

Size:10 ug

Background:Tumor necrosis factor alpha (TNF-α), also called cachectin, is the best-know member of the TNF-family, which can cause cell death. This protein is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. TNF-α occurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. The naturally-occurring form of TNF-α is glycosylated, but non-glycosylated recombinant TNF-α has comparable biological activity. The biologically active native form of TNF-α is reportedly a trimer. Human and Mouse TNF-α show approximately 79 % homology at the amino acid level and cross-reactivity between the two species. Two types of receptors for TNF-α have been described and virtually all cell types studied show the presence of one or both of these receptor types.

Research_area:Immunology

Purity:> 97 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Human Tumor Necrosis Factor-alpha, His

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by a cytotoxicity assay using Mouse L929 cells is less than .5 ng/ml, corresponding to a specific activity of > 2. × 17 IU/mg in the presence of actinomycin D.

Synonyms:TNF-α, DIF-alpha, TNFA, TNFSF2, TNLG1F, TNF, Human

Endotoxin:Less than 1 EU/μg of rHuTNF-α, His as determined by LAL method.

Storage:-20ºC

Sequence:MHHHHHHVRS SSRTPSDKPV AHVVANPQAE GQLQWLNRRA NALLANGVEL RDNQLVVPSE GLYLIYSQVL FKGQGCPSTH VLLTHTISRI AVSYQTKVNL LSAIKSPCQR ETPEGAEAKP WYEPIYLGGV FQLEKGDRLS AEINRPDYLD FAESGQVYFG IIAL

uniprot accession: P1375

Molecular weight:Approximately 18.3 kDa, a single, non-glycosylated polypeptide chain containing 164 amino acids with Met and 6 × His at N-terminus.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in PBS, pH 7..