Recombinant Human SCF/c-Kit Ligand Protein

SKU: PKSH033080

Size: 100μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: SCF;c-Kit Ligand

Target Synonym: KITLG;Kit Ligand;MGF;Mast Cell Growth Factor;SCF;Stem Cell Factor;c-Kit ligand

Research Areas: Developmental Biology;immunology;Stem cells

Conjugation:

Target Species: Human

Expression Host: E.coli

Application: Cell culture

Fusion tag: C-His

UNIProt ID: P21583

Accession: P21583

Background: Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis. SCF stimulates the proliferation of myeloid; erythroid; and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors.

Concentration:

Activity: Measure by its ability to induce TF-1 cells proliferation. The ED₅₀ for this effect is <5 ng/mL. The specific activity of recombinant human SCF is > 5 x 10⁵ IU/mg.

Sequence: Glu26-Ala189

Purity: > 98 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method.

Calculated MW: 19.4 kDa

ObservedMW: 21 kDa