Recombinant Human/Rhesus HER4/ErbB4 Protein (Fc Tag)

SKU: PKSH031650

Size: 100μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation:

Target Synonym: ERBB4;HER4;Proto-oncogene-like protein c-ErbB-4;Receptor tyrosine-protein kinase erbB-4;Tyrosine kinase-type cell surface receptor HER4;p180erbB4

Research Areas: Signal Transduction;Tags & Cell Markers;Cancer;immunology;

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application: ELISA

Fusion tag: C-hFc

UNIProt ID: Q15303

Accession: NP_005226.1

Background: ERBB4 is a single-pass type I membrane protein with multiple cysteine rich domains; a transmembrane domain; a tyrosine kinase domain; a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. ERBB4 is expressed at highest levels in brain; heart; kidney; in addition to skeletal muscle; parathyroid; cerebellum; pituitary; spleen; testis and breast. And lower levels in thymus; lung; salivary gland; and pancreas. It specifically binds to and is activated by neuregulins; NRG-2; NRG-3; heparin-binding EGF-like growth factor; betacellulin and NTAK. ERBB4 also can be activated by other factors and induces a variety of cellular responses including mitogenesis and differentiation. ERBB4 regulates development of the heart; the central nervous system and the mammary gland; gene transcription; cell proliferation; differentiation; migration and apoptosis. It is required for normal cardiac muscle differentiation during embryonic development; and for postnatal cardiomyocyte proliferation. ERBB4 also play a role on the normal development of the embryonic central nervous system; especially for normal neural crest cell migration and normal axon guidance. It is required for mammary gland differentiation; induction of milk proteins and lactation.

Concentration:

Activity: 1. Measured by its ability to bind biotinylated human Fc-NRG1 (isoform Beta1) in a functional ELISA. 2. Measured by its ability to bind biotinylated human NRG1 (isoform Beta1) in a functional ELISA. 3. Measured by its ability to bind biotinylated human NRG1 (aa 2-246)-Fc in a functional ELISA.

Sequence: Met 1-Arg649

Purity: > 85 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH7.4
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 96.6 kDa

ObservedMW: 117 kDa