Recombinant Human Rb protein

SKU: CD01007

Size:10 ug

Background:Rb encoded by the RB1 gene in humans, is expressed by retina and belongs to the etinoblastoma-associated protein family. The hole protein consists of 928 a.a. and the rHuRb fragment occupies sequence of 792-929 a.a.. Rb is a key regulator of entry into cell division that acts as a tumor suppressor. It has many functions, for example, promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C, and acts as a transcription repressor of E2F1 target genes and so on. The rHuRb is the region that rich of modified residue like phosphothreonine and N6-acetyllysine.

Research_area:Others

Purity:> 95 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Human Retinoblastoma-Associated Protein Fragment, 137a.a. with His-tag

Biological activity:

Synonyms:Rb1, OSRC, PPP1R13, RB, p15-Rb, p11-RB1, pRb, pp11, Human

Endotoxin:Less than 1 EU/μg of rHuRb137, His as determined by LAL method.

Storage:-20ºC

Sequence:MASFPSSPLR IPGGNIYISP LKSPYKISEG LPTPTKMTPR SRILVSIGES FGTSEKFQKI NQMVCNSDRV LKRSAEGSNP PKPLKKLRFD IEGSDEADGS KHLPGESKFQ QKLAEMTSTR TRMQKQKMND SMDTSNKEEK HHHHHH

uniprot accession:P64

Molecular weight:Approximately 16.5 kDa, a single non-glycosylated polypeptide chain containing 146 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in PBS, pH 7.4.