Recombinant Human PSA/KLK3 Protein (aa 25-261, His Tag)

SKU: PKSH032949

Size: 50μg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: PSA;KLK3

Target Synonym: APS;Gamma-Seminoprotein;Hk3;KLK2A1;Kallikrein-3;P-30 Antigen;PSA;Prostate-Specific Antigen;Semenogelase;Seminin

Research Areas: Signal Transduction;Tags & Cell Markers;Neuroscience;Cancer;immunology;

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application:

Fusion tag: C-His

UNIProt ID: P07288

Accession: P07288

Background: KLK3; also known as APS; is short for Prostate-specific antigen. It is a 261 aa. protein which belongs to the peptidase S1 family and Kallikrein subfamily. This protein has 5 isforms produced by alternative splicing. It is a secreted protein and can forms a heterodimer with SERPINA5 which can inhibit its activity. KLK3 is also strongly inhibited by Zn2+; 100 times more abundant in semen than in serum. This inhibition is relieved by exposure to semenogelins; which are avid zinc binders. KLK3 can hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.

Concentration:

Activity: Not validated for activity

Sequence: Ile25-Pro261

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of 50mM Tris-HCl, 10mM CaCl₂, 150mM NaCl, 0.05% Brij 35, pH 7.5.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 27.1 kDa

ObservedMW: 35 kDa