Recombinant Human PKC epsilon/PKCE Protein (His Tag)

SKU: PKSH032964

Size: 50μg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: PKC epsilon;PKCE

Target Synonym: PKCE;PRKCE;Protein Kinase C Epsilon Type;nPKC-Epsilon

Research Areas: Signal Transduction;Cardiovascular;Cancer;

Conjugation:

Target Species: Human

Expression Host: E.coli

Application:

Fusion tag: C-His

UNIProt ID: Q02156

Accession: Q02156

Background: Protein Kinase C Epsilon type is a member of the serine- and threonine-specific protein kinase family that can be activated by calcium and the second messenger diacylglycerol. Protein Kinase C Epsilon contains these domains: one AGC-kinase C-terminal domain; one C2 domain; one protein kinase domain and two phorbol-ester/DAG-type zinc fingers. Protein Kinase C Epsilon phosphorylate a variety of protein targets and has been identified to participate in diverse cellular signaling pathways. It has many different cellular functions; such as neuron channel activation; apoptosis; cardioprotection from ischemia; heat shock response; as well as insulin exocytosis. Protein Kinase C Epsilon also serves as the receptor for phorbol esters; a class of tumor promoters.

Concentration:

Activity: Not validated for activity

Sequence: Gln580-Pro737

Purity: > 90 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 1mM DTT, pH 7.4.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 19.6 kDa

ObservedMW: 20 kDa