Recombinant Human PIGR Protein (365 Ser/Gly, His Tag)

SKU: PKSH031844

Size: 100μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation:

Target Synonym: Hepatocellular Carcinoma-Associated Protein TB6;PIGR;PIgR;Poly-Ig Receptor;Polymeric Immunoglobulin Receptor

Research Areas: immunology

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application: ELISA

Fusion tag: C-His

UNIProt ID: P01833

Accession: NP_002635.2

Background: Polymeric immunoglobulin receptor, also known asPIGR, is a member of the immunoglobulin superfamilyand aFc receptor. The ectodomain of this receptor consists of five units with homology to the variable units of immunoglobulins and a transmembrane region, which also has some homology to certain immunoglobulin variable regions. PIGR is expressed on several glandular epithelia including those of liver and breast. The deduced amino-acid sequence has a length of 764 residues and shows an overall similarity of 56% and 64% with the rabbit and rat counterpart. PIGR mediates transcellular transport of polymeric immunoglobulin molecules, and thus facilitates the secretion ofIgAandIgM. During this process, a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment of PIGR.

Concentration:

Activity: Immobilized rhhuman IgM at 2 μg/ml (100 μl/well) can bind biotinylated PIGR with a linear range of 0.94-15 ng/ml. 2. When human human IgM is immobilized at 2 μg/ml (100 μl/well), PIGR inhibits 50% binding of biotinylated PIGR (0.062 μg/ml) at the concentration range of 0.03-20 μg/ml.

Sequence: Met 1-Arg 638, 365 Ser/Gly

Purity: > 97 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 69.0 kDa

ObservedMW: