Recombinant Human/Mouse/Rat BMP-2 Protein

SKU: PKSR030454

Size: 100μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: BMP-2

Target Synonym: BMP-2;BMP-2A;BMP2;BMP2A;Bone Morphogenetic Protein 2;Bone Morphogenetic Protein 2A

Research Areas: Signal Transduction;Cardiovascular;Cancer;Stem cells

Conjugation:

Target Species: Human/Mouse/Rat

Expression Host: E.coli

Application: Cell culture

Fusion tag: C-His

UNIProt ID: P12643

Accession: P12643

Background: Bone Morphogenetic Protein-2 (BMP-2) is one of the bone-growth regulatory factors that belong to the transforming growth factor-beta (TGF-beta) superfamily of proteins. BMPs are synthesized as large precursor molecules, which are cleaved by proteolytic enzymes. The active form of BMP-2 can consist of a dimer of two identical proteins or a heterodimer of two related bone morphogenetic proteins.

Concentration:

Activity: Measure by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED₅₀ for this effect is <1.0 μg/mL.

Sequence: Gln283-Arg396

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile 20 mM sodium citrate, 0.2 M NaCl, pH 3.5.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method.

Calculated MW: 13.8 kDa

ObservedMW: 14 kDa