Recombinant Human MANF protein

SKU: CD01339

Size:5 ug

Background:MANF is a secreted neurotrophic factor that is expressed in brain, neuronal and certain non-neuronal tissues. It has been shown to promote survival, growth and function of dopamine specific neurons. MANF and its structural homolog CDNF, each contain an N-terminal saposin-like lipid binding domain, and a carboxyl-terminal domain, which is not homologous to previously characterized protein structures. MANF and CDNF can prevent 6-OHDA induced degeneration of dopaminergic neurons by triggering survival pathways in a rat experimental model of Parkinson disease. Mature human MANF is 99 %, 98 % and 96 % a.a. identical to mature rat, mouse and bovine MANF respectively.

Research_area:Immunology

Purity:> 95 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Human Mesencephalic Astrocyte-Derived Neurotrophic Factor

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by a cell proliferation assay using rat C6 cells is less than 2 μg/ml, corresponding to a specific activity of > 5 IU/mg.

Synonyms:MANF, ARMET, ARP, Human

Endotoxin:Less than 1 EU/μg of rHuMANF as determined by LAL method.

Storage:-20ºC

Sequence:LRPGDCEVCI SYLGRFYQDL KDRDVTFSPA TIENELIKFC REARGKENRL CYYIGATDDA ATKIINEVSK PLAHHIPVEK ICEKLKKKDS QICELKYDKQ IDLSTVDLKK LRVKELKKIL DDWGETCKGC AEKSDYIRKI NELMPKYAPK AASARTDL

uniprot accession:P55145

Molecular weight:Approximately 18.2 kDa, a single non-glycosylated polypeptide chain containing158 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in PBS, pH 7.4.