Recombinant Human LEFTY2 Protein (His Tag)

SKU: PKSH032686

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: LEFTY2

Target Synonym: EBAF;Endometrial bleeding-associated factor;LEFTA;LEFTY2;LEFTYA;Left-right determination factor 2;Left-right determination factor A;Protein lefty-2;Protein lefty-A;TGF-beta-4;TGFB4;Transforming growth factor beta-4

Research Areas: Signal Transduction;Cancer;Stem cells

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application:

Fusion tag: N-His

UNIProt ID: O00292

Accession: O00292

Background: Left-right determination factor 2(LEFTY2) is a secreted protein which belongs to the TGF-beta family. Lefty was first identified in a screen for undifferentiated cell-specific cDNAs from the P19 mouse embryonal carcinoma cells. Its mRNA expression on the left side of the developing embryo earned the name “Lefty”. The human orthologue was initially identified as Ebaf, Endometrial bleeding associated factor. Lefty contains the six cysteine residues that are conserved among TGF-β related proteins and that are necessary to form the cysteineknot structure. Its function in patterning left-right asymmetry of the developing organ systems such as the heart and lung is consistent in all vertebrate species examined. Lefty acts as an antagonist to Nodal signaling, potentially by competing for binding to a common receptor. It may play a role in endometrial bleeding.

Concentration:

Activity: Not validated for activity

Sequence: Phe78-Pro366

Purity: > 90 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM Histidine-HCl，4% Sucrose，4% Mannitol，0.1% Tween 80，pH6.0.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 39.1 kDa

ObservedMW: 45-50 kDa