Recombinant Human ILT4/LILRB2 protein (His tag)

SKU: PDMH100418

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Others

Abbreviation:ILT4;LILRB2

Target Synonym:CD85 Antigen-Like Family Member D;CD85d;ILT-4;Immunoglobulin-Like Transcript 4;L;LIR-2;Leukocyte Immunoglobulin-Like Receptor 2;Leukocyte Immunoglobulin-Like Receptor Subfamily B Member 2;MIR-10;Monocyte/Macrophage Immunoglobulin-Like Receptor 10

Research Areas:immunology

Conjugation:

Target Species:Human

Expression Host:HEK293 Cells

Application:

Fusion tag:C-His

UNIProt ID:Q8N423

Accession:Q8N423

Background:Members of the immunoglobulin-like transcript (ILT) family are activating and inhibitory immunoreceptors whose genes are located same locus that encodes killer cell Ig-like receptors (KIR). Leukocyte Immunoglobulin-Like Receptor Subfamily B Member 2 (LIR-2) is a type I transmembrane protein. LIR-2 is expressed primarily on monocytes and dendritic cells (DC). Human LIR-2 is produced as a 598 amino acino acid precursor including a 21 aa signal sequence, a 440 aa extracellular domain (ECD), a 21 aa transmenbrane segment, and a 116 aa cytoplasmic domain. LIR-2 binds to Classical MHCI proteins. Ligation of LIR-2 incluces Tyr phosphorylation within its cytoplasmic ITIMs, a requirement for association with SHP-1. LIR-2 mediates tolerogenic DC-induced CD4+ T cell energy in vitro and in vivo.

Concentration:

Activity:Not validated for activity

Sequence:Met1-Val461

Purity: > 95% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from sterile PBS, pH 7.4.
Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin:< 1.0 EU/mg of the protein as determined by the LAL method.

Calculated MW:50.6 kDa

ObservedMW:75 kDa