Recombinant Human IL-36RA protein

SKU: CD01498

Size:2 ug

Background:Interleukin-36 receptor antagonist (IL-36RA) is a secreted protein which belongs to the interleukin 1 cytokine family (IL-1 family) and it is predominantly expressed in keratinocytes but not in fibroblasts, endothelial cells or melanocytes. IL-36RA has been reported to antagonize the biological activity of IL-36α (IL-1F6), IL-36β (IL-1F8), and IL-36γ (IL-1F9). Furthermore, it could constitute part of an independent signaling system analogous to interleukin-1 alpha (IL-1A), beta (IL-1B) receptor agonist and interleukin-1 receptor type I (IL-1R1). In addition, The receptor for IL-36RA has not been positively identified. Indirect evidence suggests it is IL-1Rrp2. Recombinant human IL-36 RA contains 154 amino acid residues and it shares 91 % a.a. sequence identity with Mouse IL-36RA.

Research_area:Immunology

Purity:> 95 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Human Interleukin-36 Receptor Antagonist Protein

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by inhibiting IL-36 beta induced IL-8 secretion by human preadipocytes is less than 1. μg/ml, corresponding to a specific activity of > 1 IU/mg in the presence of 2 ng/ml of recombinant human IL-36 beta.

Synonyms: IL36RN,FIL1, FIL1(DELTA), FIL1D, IL-36Ra, IL1F5, IL1HY1, IL1L1, IL1RP3, IL36RA, PSORP, PSORS14,, Human

Endotoxin:Less than 1 EU/μg of rHuIL-36RA as determined by LAL method.

Storage:-20ºC

Sequence:VLSGALCFRM KDSALKVLYL HNNQLLAGGL HAGKVIKGEE ISVVPNRWLD ASLSPVILGV QGGSQCLSCG VGQEPTLTLE PVNIMELYLG AKESKSFTFY RRDMGLTSSF ESAAYPGWFL CTVPEADQPV RLTQLPENGG WNAPITDFYF QQCD

uniprot accession:Q9UBH

Molecular weight:Approximately 16.8 kDa, a single non-glycosylated polypeptide chain containing 154 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in PBS, pH 7.4.