Recombinant Human IL-10/Interleukin-10 Protein

SKU: PKSH032005

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Cytokines

Abbreviation: IL-10;Interleukin-10

Target Synonym: Interleukin;RP11-262N;CSIF;GVHDS;IL-10;IL10A;TGIF;IL10;Cytokine synthesis inhibitory factor;Interleukin-10;MGC126450;MGC126451;RP11-262N9.1

Research Areas: Cancer;immunology

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application:

Fusion tag: None

UNIProt ID: P22301

Accession: P22301

Background: Interleukin 10(IL10), also known as cytokine synthesis inhibitory factor (CSIF),is a secreted protein and belongs to the IL-10 family. IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts . IL-10 is an anti-inflammatory TH2 cytokine that has a critical role in limiting the immune response to pathogens to prevent host damage. As IL-10 in produced in several T helper populations, it is proposed that it provides a feedback loop to limit the effector functions of macrophages and DCs on T cells. Once expressed, IL-10 signals through the IL-10 receptor (IL-10R) to activate STAT3. As IL-10 is a strong inhibitor of inflammation, it has become a viable biomarker for various diseases and conditions as well as a therapeutic molecule for certain conditions. In addition to elevated levels in parasitic infection, high expression levels of IL-10 are also found in retroviral infections inducing immunodeficiency. The immunosuppressive properties of IL-10 suggest a possible clinical use of IL-10 in suppressing rejections of grafts after organ transplantations.

Concentration:

Activity: Not validated for activity

Sequence: Ser19-Asn178

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 0.01 EU per μg of the protein as determined by the LAL method.

Calculated MW: 18.6 kDa

ObservedMW: 16 kDa