Recombinant Human IL-1 beta/IL1B Protein

SKU: PKSH032628

Size: 100μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Cytokines

Abbreviation: IL-1 beta;IL1B

Target Synonym: IL1F;IL-1F;pro IL1F;IL-1;IL1-BETA;IL1F2;IL1 beta;IL1B;catabolin;IL-1B;pro Catabolin;pro IL1B;pro IL1F2;pro leukocytic endogenous mediator;pro leukocytic pyrogen;pro lymphocyte activating factor;pro mononuclear cell factor;IL-1F2;IL 1B;IL-1 beta

Research Areas: Cardiovascular;immunology;metabolism

Conjugation:

Target Species: Human

Expression Host: E.coli

Application: Cell culture

Fusion tag: C-His

UNIProt ID: P01584

Accession: P01584

Background: IL1B belongs to the IL-1 family. Interleukin 1 (IL-1) is a family of polypeptide cytokines consisting of two agonists, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2) encoded by two distinct genes and perform identical biological functions. IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response. It is identified as endogenous pyrogens, and is reported to stimulate the release of prostaglandin and collagenase from synovial cells.

Concentration:

Activity: Measure by its ability to induce IL-8 secretion in HT29 cells. The ED₅₀ for this effect is <2.0 ng/mL. The specific activity of recombinant human IL-1 beta is approximately >3 x 10⁷ IU/mg.

Sequence: Ala117-Ser269

Purity: > 98 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS,pH 8.0.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method.

Calculated MW: 18.5 kDa

ObservedMW: 17 kDa